The fresh dried pollen of grape and seedless grape varieties were used as the research material. Each cultivar was stored at room temperature, 5 C, 0 C, -18 C and -40 C respectively. Sucrose 200g / L + boric acid 50mg / L + agar 8g / L as the nutrient substrate, and the comparative study on the germination and culture of the nuclear breed and the seedless cultivar. The results showed that the pollen viability decreased with the increase of storage time at different temperatures. The pollen viability decreased at -18 C and -40 , and the pollen viability decreased at the same temperature The There was a significant difference in pollen viability between different cultivars at the beginning of storage, and the pollen viability of the kernel breed was higher than that of the seedless grape.

The main reason for the formation of nano-biotechnology is due to the penetration of nanotechnology in the biological field, nanotechnology research center is the study of nano-drug carrier. Nano-drug system targeted drug delivery to achieve drug release, increase the insoluble drugs and peptide drug bio-efficiency, reduce the toxicity and application of drugs and other aspects of the development of good prospects, and thus become one of the key research in recent years’ field. Synthesis and application of nanometer drug carriers this review is presented in recent years and its application to provide a comprehensive basis for the treatment process. Describes the nature and preparation of nano-drug carrier methods, in recent years, people have been widely concerned by scholars. Compared with the nano-drug delivery, the general pharmaceutical cannot have to extend the role of drugs, strong efficacy, and the advantages of small drug response. Nano-materials, the specific surface area, surface activity, high catalytic efficiency, surface active center, adsorption capacity and other characteristics, which has many excellent features and new features.

This research aims to identify the development of research theme trends that were carried out from 1999 to 2024. Thus, the study’s results can provide recommendations regarding research themes that can be developed to meet theoretical and practical needs. Researchers use bibliometric analysis to obtain the appropriate analysis. This analysis method can be developed to support the dynamic development of public health science with settings and researchers from developing countries, both through quantitative and qualitative interpretation. The analysis results show that over 25 years, public health science, from the perspective of researchers and developing countries, has experienced dynamic development. This change was driven by the emergence of various issues in society itself. For example, the 1999–2009 shows that lifestyle changes have resulted in multiple diseases. In the following period, the concept of sustainability emerged, which encouraged awareness of sustainable development and resource scarcity that would affect public health quality. As for the 2020–2024 period, the emergence of Covid 19 changed the previous research paradigm.