Objective: To determine the presence of bacteria by means of microbiological analysis on the surfaces contacted by the operator during the taking and processing of intraoral radiographs at different times of the day in the Oral Radiology Service of the UPCH. Materials and methods: Nine surfaces of the oral radiology service were sampled. The samples were taken at two times by the same investigator; at the beginning and the end of the activities in the service, the surfaces were swabbed with Trypticase Soy Broth (TSB). The samples were inoculated and incubated in three culture media (Plate Count Agar, Lamb’s Blood Agar and Cetrimide Agar). Then the respective Colony Forming Unit (CFU) count was performed and Gram staining was also performed. Results: A high concentration of bacteria (4180 CFU/mL) and fungi was found in the oral radiology service. Gram-positive cocci were the most frequently found microorganisms and gram-negative bacilli were less frequently found. Conclusions: There is a high contamination of bacteria in the oral radiology service. When the activities are completed, the number of bacteria decreases, but the variety of bacteria increases.

A topic of current interest in forestry science concerns the regeneration of degraded forests and areas. Within this topic, an important aspect refers to the time that different forests take to recover their original levels of diversity and other characteristics that are key to resume their functioning as ecosystems. The present work focuses on the premontane rainforests of the central Peruvian rainforest, in the Chanchamayo valley, Junín, between 1,000 and 1,500 masl. A total of 19 Gentry Transects of 2 × 500 m, including all woody plants ≥2.5 cm diameter at breast height were established in areas of mature forests, and forests of different ages after clear-cutting without burning. Five forest ages were considered, 5-10, 20, 30, 40 and ≥50 years. The alpha-diversity and composition of the tree flora under each of these conditions was compared and analyzed. It was observed that, from 40 years of age, Fisher’s alpha-diversity index becomes quite similar to that characterizing mature forests; from 30 years of age, the taxonomic composition by species reached a similarity of 69–73%, like those occurring in mature forests. The characteristic botanical families, genera and species at each of the ages were compared, specifying that as the age of the forest increases, there are fewer shared species with a high number of individuals. Early forests, up to 20 years of age, are characterized by the presence of Piperaceae; after 30 years of age, they are characterized by the Moraceae family.

There are numerous studies reported on the usage of the sapindus emarginatus (SE) fruit in cancer and other treatments in the past few years. In this study, crude SE fruit extract was prepared and it was further used to synthesis gold nanoparticles (Au Nps). The synthesized Au Nps were left embedded in the SE fruit extract. The Au Nps embedded in the SE fruit extract (SE-Au Nps) were characterized using UV-Visiable Spectroscopy, Centrifugal Particle Size analyzer (CPS), Scanning Electron Microscope (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). MTT assay was carried out for both SE fruit extract and SE-Au Nps on MCF7 breast cancer cell line and thus compared. The UV-Visible Absorbance for the SE-Au Nps was obtained at 543 nm. The centrifugal particle size analysis of the Au Nps embedded in SE fruit extract showed the size of the nanoparticles to be widely varying with higher fraction of particles between the size ranges of 15 to 20 nm. The morphology of the Au Nps embedded in SE fruit extract was observed using SEM. The presence of Au Nps in SE fruit extract was confirmed using FTIR. The results of the MTT assay on MCF7 breast cancer cell line proved that the % cell viability was less for SE-Au Nps than that of the SE fruit extract alone. Thus, the antiproliferative activity of the SE fruit extract was significantly enhanced by embedding it with Au Nps and it can be effectively used in therapeutic applications after further studies.