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Green synthesis of selenium nanoparticles using the aqueous extract of Catharanthus roseus (L.) leaves and evaluation of their anticancer activity
Hai Tu Le
Lan Anh Thi Nguyen
Tuyet Anh Thi Le
Characterization and Application of Nanomaterials 2026, 9(3), 026260023; https://doi.org/10.24294/can026260023
Submitted:07 Jun 2026
Accepted:28 Jul 2026
Published:31 Aug 2026
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Cite This Article
Abstract
Selenium nanoparticles (SeNPs) have attracted considerable interest for their promising biomedical applications, particularly in cancer therapy. In this study, an environmentally friendly approach was developed to synthesize SeNPs using an aqueous leaf extract of Catharanthus roseus (L.), which served as both the reducing and stabilizing agent for converting H₂SeO₃ into elemental selenium nanoparticles. The extraction process was optimized at a leaf-to-water ratio of 20 g per 100 mL of double-distilled water, with boiling for 70 min. The optimal conditions for SeNP synthesis were 12 mL of leaf extract mixed with 30 mL of 5 mM H₂SeO₃ at pH 8 and 80 °C for 105 min. Morphological analysis revealed that the biosynthesized SeNPs were predominantly spherical, with an average particle size of approximately 50 nm. GC–MS and FTIR analyses demonstrated the presence of phytochemicals in the leaf extract that participated in nanoparticle formation while providing surface capping and stabilization. The biological activity of the synthesized SeNPs was evaluated against SK-LU-1, Hep-G2, and MCF-7 cancer cell lines. After 72 h of treatment, the nanoparticles exhibited potent cytotoxicity, with half-maximal inhibitory concentration (IC₅₀) values of 5.54 μg/mL, 4.45 μg/mL, and 4.93 μg/mL, respectively. These findings indicate that Catharanthus roseus (L.)-mediated SeNPs represent a simple, sustainable, and effective nanomaterial with promising potential for anticancer applications.
References
1.Mumtaz A, Mohammad A, Ahmad S. Role of natural products in green synthesis of nanoparticles. In: Green Biosynthesis of Nanoparticles Mechanisms and Applications. Printed and Bound in the UK by Berforts Information Press Ltd. 2013; 31-52.
2.Mittal K, Chisti Y, Banerjee C. Synthesis of metallic nanoparticles using plants. Biotechnology Advances. 2013; 31, 346-356.
3.Salem S, Fouda A. Green Synthesis of Metallic Nanoparticles and Their Prospective Biotechnological Applications: An Overview. Biological Trace Element Research. 2020.
4.Karthik K, Binoy C, Rajeshkumar S, et al. A review on selenium nanoparticles and their biomedical applications. Biomedical Technology. 2024; 6, 61-74.
5.Ramya S, Shanmugasundaram T, Balagurunathan R. Biomedical potential of actinobacterially synthesized selenium nanoparticles with special reference to anti-biofilm, anti-oxidant, wound healing, cytotoxic and antiviral activities. Journal of Trace Elements in Medicine and Biology. 2015; 32, 30-39.
6.Alam H, Khatoon N, Raza M, et al. Synthesis and characterization of nano selenium using plant biomolecules and their potential applications. BioNanoScience. 2019; 9(1), 96-104.
7.Hassanien R, Abed‐Elmageed A, Husein Z. Eco‐Friendly Approach to Synthesize Selenium Nanoparticles: Photocatalytic Degradation of Sunset Yellow Azo Dye and Anticancer Activity. ChemistrySelect. 2019; 4(31), 9018- 9026.
8.Prashant K, Suresh G, Trupti P, et al. Plant extract assisted eco-benevolent synthesis of selenium nanoparticles—A review on plant parts involved, Characterization and their recent applications, Journal of Chemical Reviews. 2020; 2(3), 157-168. doi:10.22034/jcr.2020.106601
9.Tripathi M, et al. Biosynthesis of highly stable fluorescent selenium nanoparticles and the evaluation of their photocatalytic degradation of dye. BioNanoScience. 2020; 1-8.
10.Tianyu L, Huaping X. Selenium-Containing Nanomaterials for Cancer Treatment. Cell Reports Physical Science. 2020; 100111, July 22.
11.Praveen S, Ravikanth N, Pawan G, et al. Improved extraction of intracellular biogenic selenium nanoparticles and their specificity for cancer chemoprevention. Journal of Nanomedicine & Nanotechnology. 2014; 5:2, 1000194.
12.Ali N, Sawsan M, Fatma S. Evaluation of selenium nanoparticles as a potential chemopreventive agent against lung carcinoma. International Journal of Pharmaceutical Biological and Chemical Sciences. 2013; 2(4), 38-46.
13.Sasidharan S, Sowmiya R, Balakrishnaraja R. Biosynthesis of selenium nanoparticles using citrus reticulata peel extract. World Journal of Pharmaceutical Research. 2015; 4(1), 1322-1330.
14.Sharma G. Biomolecule-mediated synthesis of selenium nanoparticles using dried Vitis vinifera (raisin) extract. Molecules. 2014; 19(3), 2761-2770.
15.Deepa B, Ganesan V. Bioinspiredsynthesis of selenium nanoparticles using flowers of Catharanthus roseus (L.) G. Don. and Peltophorum pterocarpum (DC.) Backer ex Heyne—a comparison. International Journal of ChemTech Research. 2015; 7(2), 725-733.
16.Jay V, Shafkat R. Green synthesis of selenium nanoparticles using Allium sativum extract. Asian Journal of Life sciences. 2017; 6(3), 436-440.
17.Anu K, Singaravelu G, Murugan K. Green-synthesis of selenium nanoparticles using garlic cloves (Allium sativum): biophysical characterization and cytotoxicity on vero cells. Journal of Cluster Science. 2017; 28(1), 551-563.
18.Vyas J, Rana S. Antioxidant activity and green synthesis of selenium nanoparticles using allium sativum extract. International Journal of Phytomedicine. 2017; 9, 634.
19.Thangavelu S, Periyakali Saravana B and Subramaniyam K. Green synthesis of selenium nanoparticles from sodium selenite using garlic extract and its enrichment on Artemia nauplii to feed the freshwater prawn Macrobrachium rosenbergii postlarvae. Research Journal of Chemistry and Environment. 2017; 21(10), 1-12.
20.Jay V and Shafkat R. Synthesis of seleniumnanoparticles using Allium sativum extract and analysis of their antimicrobial property against gram positive bacteria. The Pharma Innovation Journal. 2018; 7(9): 262-266.
21.Vyas J, Rana H. Antioxidant activity and biogenic synthesis of selenium nanoparticles using the leaf extract of aloe vera. International Journal of Current Pharmaceutical Research. 2017; 9, 147-152.
22.Kapur M, Soni K, Kohli K. Green synthesis of selenium nanoparticles from broccoli, characterization, application and toxicity. Advanced Techniques in Biology & Medicine. 2017; 5(1), 2379-1764.
23.Dongxiao C, Tingting L, Liquian S et al. Green synthesis of selenium nanoparticles with extract of hawthornfruit induced HepG2 cells apoptosis. Pharmaceutical biology. 2018; 56(1), 528-534.
24.Sivakumar C, Jeganathan K. In-vitro cytotoxicity of java tea mediated selenium nanoballs against L6 cell lines. Journal of Drug Delivery and Therapeutics. 2018; 8(6), 195-200.
25.Mellinas C., Jiménez A, Garrigós C. Microwave-Assisted Green Synthesis and Antioxidant Activity of Selenium Nanoparticles Using Theobroma cacao L. Bean Shell Extract. Molecules. 2019; 24(22), 4048.
26.Soumya M, Shrudhi S, Happy A, et al. Efficacy of Biogenic Selenium Nanoparticles from an extract of ginger towards evaluation on anti-microbial and antioxidant activities. Colloid and Interface Science Communications. 2019; 29, 1-8.
27.Dabei F, Li L, Zhizhen L, et al. Biosynthesis of selenium nanoparticles and their protective, antioxidative effects in streptozotocin induced diabetic rats. Science and Technology of Advanced Materials. 2020; 21(1), 505-514.
28.Nayeem M, Sachin O, Raghvendra B. Rapid and sizecontrolled biosynthesis of cytocompatible selenium nanoparticles by Azadirachta indica leaves extract for antibacterial activity. Materials Letters. 2020; 264, 127353.
29.Vetrivel C, Durairaj K, Kalaimurugan D. Green synthesis of selenium nanoparticles mediated from Ceropegia bulbosa Roxb extract and its cytotoxicity, antimicrobial, mosquitocidal and photocatalytic activities. Scientific Reports. 2021; 11:1032. doi: 10.1038/s41598-020-80327-9.
30.Ecem E, Aydın A. Selenium nanoparticles synthesized via green methods from Calluna vulgaris extract: Exploring their antioxidant and antibacterial activities. International Journal of Secondary Metabolite. 2024; 11(3), 462–471. doi: 10.21448/ijsm.1415795.
31.Georgia B, Sandra S, Genickson J, et al. Green synthesis of selenium nanoparticles mediated aqueous extract of Euphorbia Tirucalli and its antimicrobial and cytotoxic activities. African Journal of Biomedical Research. 2024; 27(3); 1035-1042. doi: 10.53555/AJBR.v27i3.2748.
32.Krystyna P. Plant extracts for production of functionalized selenium nanoparticles. Materials. 2024; 17, 3748. doi: 10.3390/ma17153748.
33.Hua C , Li W, Shuqing J, et al. Green synthesis of selenium nanoparticles by grape seed extract synergized with ascorbic acid: Preparation optimization, structural characterization, and functional activity. Foods. 2025; 14, 3002, 1-17. doi: 10.3390/foods14173002.
34.Le S , Man L, Yingxiu W, et al. Biosynthesis of selenium nanoparticles from Rosa rugosa extract: Mechanisms and applications for sustainable crop protection. Agronomy. 2025; 15, 2385. doi: 10.3390/agronomy15102385.
35.Mohamed E, Heba A, Gehan A, et al. Eco-friendly synthesis of Balanites aegyptiaca-derived selenium nanoparticles: extract and assessment of their anticancer, antimicrobial, cytogenetic and molecular docking insights. Scientific Reports. 2026; 16: 4721. doi: 10.1038/s41598-026-35358-z.
36.Sama H, Nada K, Esraa O, et al. Facile green synthesis of selenium nanoparticles using olive (Olea europaea) leaf extract and their antimicrobial and antibiofilm properties. Scientific Reports. 2026; 16:15224. doi: 10.1038/s41598-026-47329-5.
37.Halvin A, Subramani K. Broccoli-mediated green synthesis of selenium nanoparticles with potent antioxidant and antimicrobial activities: Experimental and molecular docking insights. Food Chemistry Advances. 2026; 10, 101260. doi: 10.1016/j.focha.2026.101260.
38.Aya E, Amr M and Heba A. Biosynthesis of selenium nanoparticles by Aloe vera leaf extract and its biomedical applications. Discover Nano. 2026; 21:91. doi: 10.1186/s11671-026-04489-7.
39.Anjali S and Sheetal S. Phytochemical analysis and free radical scavenging potential of herbal and medicinal plant extracts. Journal of Pharmacognosy and Phytochemistry. 2013; 2(4), 22–29.
40.Skehan P, Storeng R, Scudiero D, et al. New colorimetric cytotoxicity assay for anticancerdrug screening, Journal of National Cancer Institute. 1990, 82, pp.1107–1112.
41.Isaac G, Gabriela T, Daniel U, et al. Green synthesis of silver nanoparticles with phytosterols and betalain pigments as reducing agents present in cactus. Myrtillocactus geometrizans. 2020; 5, 3361–3369.
42.Su W, Yang H, Wang C. Biosynthesis and antioxidation of nano-selenium using lemon juice as a reducing agent. Green Processing and Synthesis. 2021; 10: 178–188.
43.Nahid S, Saba Z, Fatemesh K. Selenium nanoparticles synthesis, invitro cytotoxicity, antioxidant activity and interaction studies with ct-DNA and HAS, HHb and Cyt c serum proteins. Biotechnology Reports. 2021; 30, e00615.
44.Mustafa M. El-Z, Mostafa M. E, Hani A, et al. The antimicrobial, antioxidant, and anticancer activity of greenly synthesized selenium and zinc composite nanoparticles using Ephedra aphylla extract. Biomolecules. 2021; 11, 470, 1-17.
45.Naiyer S, Wajahatullah K. Phytosterols as a natural anticancer agent: Current status and future perspective, Biomedicine & Pharmacotherapy. 2017; 88, 786-794.
46.Shenbagamoorthy S, Ramar T, Vellingiri S, et al. γ-Sitosterol from Acacia nilotica L. induces G2/M cell cycle arrest and apoptosis through c-Myc suppression in MCF-7 and A549 cells. Journal of Ethnopharmacology. 2012; 141(3), 803-809.
47.Wen W, Shih C, Yueh L, et al. Stigmasterol inhibits cancer stem cell activity in endometrial cancer by repressing IGF1R/mTOR/AKT pathway, Journal of Functional Foods. 2022; 99,105338, 1-12.
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