This study, through the method of canonical correlation analysis, revealed significant correlations between various dimensions of learning attitudes of students and various dimensions of teacher knowledge. An analysis of data from a group of 221 high school students showed that teacher knowledge of teaching content, theoretical knowledge, and teaching practice and classroom management significantly impact learning attitudes of students. Specifically, teacher knowledge of teaching content plays a crucial role in promoting students’ behavioral inclination to learn chemistry, teachers’ theoretical knowledge significantly enhances students’ liking for chemistry laboratory courses, while teachers’ teaching practice and classroom management have a suppressive effect on students’ evaluative beliefs about school chemistry. The results of this study provide effective guidance for both the theory and practice of high school chemistry education.