This work shows the results of the biosynthesis of silver nanoparticles using the microalga Chlorella sp, using growth media with different concentrations of glycerol, between 5%–20%, and different light and temperature conditions. The synthesis of nanoparticles was studied using supernatants and pellets from autotrophic, heterotrophic and mixotrophic cultures of the microalga. The presence of nanoparticles was verified by ultraviolet-visible spectroscopy and the samples showing the highest concentration of nanoparticles were characterized by scanning electron microscopy. The mixotrophic growth conditions favored the excretion of exopolymers that enhanced the reduction of silver and thus the formation of nanoparticles. The nanoparticles obtained presented predominantly ellipsoidal shape with dimensions of 108 nm × 156 nm and 87 nm × 123 nm for the reductions carried out with the supernatants of the mixotrophic cultures with 5% and 10% glycerol, respectively.
The boom in nanotechnology over the last three decades is undeniable. Responsible for this interest in nanomaterials are mainly the nanostructured forms of carbon, since historically they were the ones that inaugurated the study of nanomaterials with the discovery of fullerenes in 1985 and carbon nanotubes in 1991. Although a variety of techniques exist to produce these materials, chemical vapor deposition (CVD) is particularly valuable as it allows the production of a wide variety of carbon nanostructures, is versatile, scalable, easy to implement and relatively low cost. This review article highlights the importance of CVD and details its principles, operating conditions and parameters, as well as its main variants. A description of the technique used to produce fullerenes, nano-ceramics, carbon nanotubes, nanospheres, graphene and others is made, emphasizing the specific parameters for each synthesis.
Different color-promoting treatments were tested on table grape cv. “Flame Seedlees” to evaluate changes on flavonoids such as anthocyanins and the residual ethylene produced. Treatments were spray-applied at the onset of veraison. The control was Ethrel at 250 ppm (ETH), Salicylic Acid at 100 ppm (AS), Melatonin at 25 ppm (MEL) and 1:1 mixtures of ETH+AS, ETH+MEL and AS+MEL. The trials were conducted in triplicate after harvest, measuring Total Soluble Solids (% TSS), total acidity (% tartaric acid), pH, residual ethylene (ppm) and anthocyanin content (mg∙cm-2). It was found that treatments ETH, AS, MEL and ETH+AS reached 16% TSS, standing out with lower values ETH +MEL (14.27%) and AS+MEL (15.17%) (p ≤ 0.05). ETH reached 0.83 ppm of residual ethylene, while a sum effect was appreciated in ETH+AS (0.5 ppm) and ETH+MEL (0.35 ppm), but not beneficial as it did not reflect quality characteristics. Only differences (p ≤ 0.05) in anthocyanin content were recorded between ETH (0.019 mg∙cm-2) and AS+MEL (0.003 mg∙cm-2). The subjective color of the grape bunches in the field made it possible to relate it to the objective results of the analyses performed. This research provides commercially important information on the substitution of Ethrel by natural compounds such as AS and MEL, as they show similar effects on the quality of “Flame Seedless” table grapes. In addition, these compounds do not have an ethylene residual greater than 0.2 mg/kg.
With the purpose of knowing the phytosocilogy of weeds associated to a carrot crop (Daucus carota L.) under conditions of the municipalities of Ventaquemada and Jenesano-Boyacá, one lot per municipality destined to carrot cultivation was selected and a W-shaped layout was made covering an area of 500 m2. Relative density, relative frequency, relative dominance and the importance value index (IVI) were calculated, as well as the Alpha and Beta diversity indices for the sampled areas. A total of 6 families and 11 species were counted, of which 63.64% were represented by annual plants and 36.36% by perennial plants. The class Liliopsida (Monocotyledon) was represented by the Poaceae family. The Magnoliopsida class (Dicotyledon) was represented by the following families: Asteraceae, Brassicaceae, Boraginaceae, Leguminosaceae, Polygonaceae, the last one being the one with the highest number of species. The species R. crispus and P. nepalense were the ones with the highest values of Importance Value Index (IVI) with 0.953 and 0.959, respectively. According to the Shannon-Wiener diversity and Simpson’s dominance indices, the evaluated areas presented a low species diversity and a high probability of dominant species. The results obtained can serve as a basis and tool for carrot growers in the evaluated areas to define management plans for the associated weeds and thus optimize yields in this crop.
Introduction: The selection of genotypes with determinate growth habit in tomato should contemplate adequate selection criteria to increase the efficiency of the breeding program. Objective: The objective of this work was to estimate selection criteria for “chonto” type tomato lines with determined growth habit. Materials and methods: This work was carried out at the Universidad Nacional de Colombia (Palmira Campus), in 2016, with seven lines with determinate growth habit and a control with indeterminate growth. Heritability in a broad sense (h2 g), coefficient of environmental variation, coefficient of genetic variation, selection efficiency and genetic gain were determined in parameters of morphological, phonological, fruit quality, fruit shape and production, using the RELM/BLUP procedure of the SELEGEN software. Results: There were three ranges of h2 g, the first with values of h2 g greater than 0.76, the second between 0.53 and 0.38, and the third with a value less than 0.38. The highest values of h2 g were for final plant height with 0.92, plant height at harvest with 0.88, yield per plant with 0.83, days to flowering with 0.83, number of fruits per plant with 0.82, and days to harvest with 0.82. For genetic gain it was found that the control had the highest values for final plant height, plant height at harvest, internode length, days to harvest, harvest duration, soluble solids content, number of fruits per plant, fruit weight and yield per plant; however, in some parameters such as height and phenology for selection by determined growth habit, the lowest values were better. Conclusion: There was evidence of genetic parameters that could be considered as selection criteria for “chonto” type tomato lines with determinate growth habit.
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