There are numerous studies reported on the usage of the sapindus emarginatus (SE) fruit in cancer and other treatments in the past few years. In this study, crude SE fruit extract was prepared and it was further used to synthesis gold nanoparticles (Au Nps). The synthesized Au Nps were left embedded in the SE fruit extract. The Au Nps embedded in the SE fruit extract (SE-Au Nps) were characterized using UV-Visiable Spectroscopy, Centrifugal Particle Size analyzer (CPS), Scanning Electron Microscope (SEM) and Fourier Transform Infrared Spectroscopy (FTIR). MTT assay was carried out for both SE fruit extract and SE-Au Nps on MCF7 breast cancer cell line and thus compared. The UV-Visible Absorbance for the SE-Au Nps was obtained at 543 nm. The centrifugal particle size analysis of the Au Nps embedded in SE fruit extract showed the size of the nanoparticles to be widely varying with higher fraction of particles between the size ranges of 15 to 20 nm. The morphology of the Au Nps embedded in SE fruit extract was observed using SEM. The presence of Au Nps in SE fruit extract was confirmed using FTIR. The results of the MTT assay on MCF7 breast cancer cell line proved that the % cell viability was less for SE-Au Nps than that of the SE fruit extract alone. Thus, the antiproliferative activity of the SE fruit extract was significantly enhanced by embedding it with Au Nps and it can be effectively used in therapeutic applications after further studies.

In order to evaluate the temporal changes in tree diversity of forest vegetation in Xishuangbanna, Yunnan Province, the study collected tree diversity data from four main forest vegetation in the region through a quadrat survey including tropical rainforest (TRF), tropical coniferous forest (COF), tropical lower mountain evergreen broad-leaved forest (TEBF), tropical seasonal moist forest (TSMF). We extracted the distribution of four forest vegetation in the region in four periods of 1992, 2000, 2009, and 2016 in combination with remote sensing images, using simp son Shannon Wiener and scaling species diversity indexes compare to the differences of tree evenness of four forest vegetation and use the scaling ecological diversity index and grey correlation evaluation model to evaluate the temporal changes of forest tree diversity in the region in four periods. The results show that: (1) The proportion of forest area has a trend of decreasing first and then increasing, which is shown by the reduction from 65.5% in 1992 to 53.42% in 2000, to 52.49% in 2009, and then to 54.73% in 2016. However, the tropical rainforest shows a continuous decreasing trend. (2) There are obvious differences in the contributions of the four kinds of forest vegetation to tree diversity. The order of evenness is tropical rainforest > tropical mountain (low mountain) evergreen broad-leaved forest > warm coniferous forest > tropical seasonal humid forest, and the order of richness is tropical rainforest > tropical mountain (low mountain) evergreen broad-leaved forest > tropical seasonal humid forest > warm coniferous forest, The order of contribution to tree diversity in tropical rainforest > tropical mountain (low mountain) evergreen broad-leaved forest > tropical seasonal humid forest > warm tropical coniferous forest. (3) The tree diversity of tropical rainforests and tropical seasonal humid forests showed a continuous decreasing trend. The tree diversity of forest vegetation in Xishuangbanna in four periods was 1992 > 2009 > 2016 > 2000. The above results show that economic activities are an important factor affecting the biodivesity of Xishuangbanna, and the protection of tropical rainforest is of great significance to maintain the biodiversity of the region.

Objective: To determine the presence of bacteria by means of microbiological analysis on the surfaces contacted by the operator during the taking and processing of intraoral radiographs at different times of the day in the Oral Radiology Service of the UPCH. Materials and methods: Nine surfaces of the oral radiology service were sampled. The samples were taken at two times by the same investigator; at the beginning and the end of the activities in the service, the surfaces were swabbed with Trypticase Soy Broth (TSB). The samples were inoculated and incubated in three culture media (Plate Count Agar, Lamb’s Blood Agar and Cetrimide Agar). Then the respective Colony Forming Unit (CFU) count was performed and Gram staining was also performed. Results: A high concentration of bacteria (4180 CFU/mL) and fungi was found in the oral radiology service. Gram-positive cocci were the most frequently found microorganisms and gram-negative bacilli were less frequently found. Conclusions: There is a high contamination of bacteria in the oral radiology service. When the activities are completed, the number of bacteria decreases, but the variety of bacteria increases.