The study evaluated the aseptic establishment of Monstera acuminata Koch and Monstera deliciosa Liebm (Araceae) from leaves and the induction of in vitro organogenesis of M. acuminata K. from stem discs of young shoots. For this purpose, different disinfection protocols were applied to mature leaves and young shoots, from which leaf explants of approximately 1 cm2 and stem discs of approximately 1 mm thickness were extracted. The explants were established in semi-solid media with different hormone treatments during the aseptic establishment stage and induction of organogenesis. Disinfection with 3% sodium hypochlorite (NaClO) for 20 min and 50% Murashige and Skoog[1] medium with plant tissue culture preservative (PPM) favored less oxidation in leaf explants of both species. All explants of M. deliciosa in both treatments grown in PPM-added medium and at different disinfection protocols survived, showed no contamination and more than 80% retained cellular activity up to 49 days of culture age. At 35 days of culture, with disinfection in Tween-20 + 20% ethanol + 2.5% NaClO, and seeding of explants in MS medium added with 1 mg/L of BAP, 0.5 mg/L of AIA and 0.1 mg/L of ANA, seven new shoots of stem discs were induced. Monstera deliciosa was more adaptable to in vitro conditions. Advances in aseptic establishment and induction of organogenesis in native Araceae for wicker production are the basis for ex situ conservation of local populations.
The use of saline water in agriculture is a viable alternative, considering the increased demand for fresh water. The objective of this study was to evaluate the growth and phytomass production of sugar beet under irrigation with water of different saline concentrations in a field experiment on the campus of the Federal University of Alagoas in Arapiraca. The treatments were five levels of electrical conductivity (1.0, 2.0, 3.0, 4.0 and 5.0 dS m-1). The design was in randomized blocks, with four repetitions. The maximum yield of sugar beet at 27 days after the application of saline treatments was obtained with a salinity of 3.0 dS m-1, for the variables plant height (PA), stem diameter (CD), root length (RC), aboveground dry phytomass (FSPA) and total dry phytomass (FST). At 42 days after the application of saline treatments, the variables aboveground fresh phytomass (FFPA), root fresh phytomass (FFR), total fresh phytomass (FFT), aboveground dry phytomass (FSPA) and total dry phytomass (FST) increased with increasing water salinity. Rain may have influenced the results obtained for the evaluations, performed at 42 days after the application of the saline treatments.
Humic substances are used in agriculture as promoters of plant growth, especially of the root system. The objective of the work was to evaluate the effect of the application of different doses of fulvic acid on the growth and productivity of American lettuce, Raider Plus cultivar. The experimental design used was entirely randomized, with five treatments of fulvic acid 0, 1, 2, 4, 8 mL·L-1 and four repetitions, applied at the time of transplanting. Two experiments were conducted simultaneously: one in the greenhouse, where fresh and dry mass of the aboveground and root parts, length and volume of the roots were evaluated; and the other in the field, where, at the end of the cycle, fresh and dry mass of the aboveground parts, number of leaves, stem length and average head circumference were evaluated. The application of different doses of fulvic acid promoted the growth of lettuce plants, especially the root system. The emission of roots, with predominance, of those of smaller diameter, was found in the higher concentrations of fulvic acid. The number of leaves and the average circumference of the head expressed responses in the concentrations of fulvic acid.
This paper examines the transformative potential of e-government in public administration, focusing on its capacity to enhance service delivery, transparency, accessibility, cost efficiency, and civic engagement. The study identifies key challenges, including inadequate technological infrastructure, cybersecurity vulnerabilities, resistance to change within public institutions, and a lack of public awareness about e-government services. These barriers hinder the seamless operation and adoption of digital government initiatives. Conversely, the study highlights significant opportunities such as streamlined service delivery, enhanced transparency through real-time access to government data, increased accessibility for marginalized and remote communities, substantial cost savings, and greater civic engagement via digital platforms. Addressing these challenges through targeted strategies—enhancing technological infrastructure, bolstering cybersecurity, managing organizational change, and raising public awareness—can help policymakers and public administrators implement more effective and inclusive e-government initiatives. Additionally, the integration of these digital solutions can drive sustainable development and digital inclusion, fostering social equity and economic growth. By leveraging these opportunities, governments can achieve more efficient, transparent, and accountable governance. Ultimately, the successful implementation of e-government can transform the relationship between citizens and the state, building trust and fostering a more participatory democratic process.
Background: Multiple sclerosis is often a longitudinal disease continuum with an initial relapsing-remitting phase (RRMS) and later secondary progression (SPMS). Most currently approved therapies are not sufficiently effective in SPMS. Early detection of SPMS conversion is therefore critical for therapy selection. Important decision-making tools may include testing of partial cognitive performance and magnetic resonance imaging (MRI). Aim of the work: To demonstrate the importance of cognitive testing and MRI for the prediction and detection of SPMS conversion. Elaboration of strategies for follow-up and therapy management in practice, especially in outpatient care. Material and methods: Review based on an unsystematic literature search. Results: Standardized cognitive testing can be helpful for early SPMS diagnosis and facilitate progression assessment. Annual use of sensitive screening tests such as Symbol Digit Modalities Test (SDMT) and Brief Visual Memory Test-Revised (BVMT-R) or the Brief International Cognitive Assessment for MS (BICAMS) test battery is recommended. Persistent inflammatory activity on MRI in the first three years of disease and the presence of cortical lesions are predictive of SPMS conversion. Standardized MRI monitoring for features of progressive MS can support clinically and neurocognitively based suspicion of SPMS. Discussion: Interdisciplinary care of MS patients by clinically skilled neurologists, supported by neuropsychological testing and MRI, has a high value for SPMS prediction and diagnosis. The latter allows early conversion to appropriate therapies, as SPMS requires different interventions than RRMS. After drug switching, clinical, neuropsychological, and imaging vigilance allows stringent monitoring for neuroinflammatory and degenerative activity as well as treatment complications.
Cardiovascular imaging analysis is a useful tool for the diagnosis, treatment and monitoring of cardiovascular diseases. Imaging techniques allow non-invasive quantitative assessment of cardiac function, providing morphological, functional and dynamic information. Recent technological advances in ultrasound have made it possible to improve the quality of patient treatment, thanks to the use of modern image processing and analysis techniques. However, the acquisition of these dynamic three-dimensional (3D) images leads to the production of large volumes of data to process, from which cardiac structures must be extracted and analyzed during the cardiac cycle. Extraction, three-dimensional visualization, and qualification tools are currently used within the clinical routine, but unfortunately require significant interaction with the physician. These elements justify the development of new efficient and robust algorithms for structure extraction and cardiac motion estimation from three-dimensional images. As a result, making available to clinicians new means to accurately assess cardiac anatomy and function from three-dimensional images represents a definite advance in the investigation of a complete description of the heart from a single examination. The aim of this article is to show what advances have been made in 3D cardiac imaging by ultrasound and additionally to observe which areas have been studied under this imaging modality.
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