The H3N2 influenza virus is spiking dramatically, which is a major concern worldwide and in India. The multifunctional hetero-trimer influenza virus RNA-dependent RNA polymerase (RdRP) is involved in the generation of viral mRNA and is crucial for viral infectivity, which is directly related to the virus’s ability to survive. The goal of the current work was to use molecular docking to determine how the RdRP protein might be affected by powerful bioactive chemicals found in Calotropis gigantia latex. By applying CB-dock 2 analysis and 2D interactions, an in-silico docking study was conducted using a GC-FID (gas chromatography with flame-ionization detection) based composition profile. Tocospiro A (15%), Amyrin (7%), and Gombasterol A were found by GC-FID to be the main phytocompounds in the latex of Calotropis gigantia. The docking result showed that ligands were effectively bound to RdRP. According to interaction studies, RdRP/ligand complexes create hydrogen bonds, van der Waals forces, pi-alkyl bonds, alkyl bonds, and pi-Sigma bonds. Therefore, it was suggested that Calotropis gigantia latex may represent a possible herbal remedy to attenuate H3N2 infections based on the above findings of the fragrance profile and docking.

This multiple case study qualitative research examined the impact of adoption and diffusion of innovation on Small and Medium Enterprises (SME’s) growth in the hostile business landscape of Khyber-Pakhtunkhwa, Pakistan. This research is intended to investigate research data and consequent findings based on an interview protocol that was purposefully developed from extant literature, complemented by an initial pilot study of two pharmaceutical SMEs. The researcher conducted 20 interviews, guided by the semi-structured interview protocol offered to the respondents beforehand after sorting their informed consent. The 20 participants represented the different hierarchal levels of the 08 case study of pharmaceutical from the two industrial clusters of Khyber Pakhtunkhwa, Pakistan, located at the Hayatabad Industrial Estate, Peshawar, and the Rashkai Industrial Estate, Nowshera. The analysis of the data presented findings and corroborated the research propositions that those SMEs that are structurally entrepreneurial and adopt innovation amenably, are open to mobility and tourism, yield satisfactory results in terms of their growth as compared to those that are inertial and unentrepreneurial. Similarly, the results offer confirmation that the effectiveness of government agencies that are explicitly formed to address the problems of small businesses is insufficient. They rather create hindrances than assistance due to the excessive delays in approving innovative ideas and conceptions by these related organizations and ministries. Moreover, the proposed framework offers pragmatic recommendations to contextualize entrepreneurial culture and innovative structures in SMEs and their essential factors in critical environmental circumstances.

Modelling and simulation have now become standard methods that serve to cut the economic costs of R&D for novel advanced systems. This paper introduces the study of modelling and simulation of the infrared thermography process to detect defects in the hydroelectric penstock. A 3-D penstock model was built in ANSYS version 19.2.0. Flat bottom holes of different sizes and depths were created on the inner surface of the model as an optimal scenario to represent the subsurface defect in the penstock. The FEM was applied to mimic the heat transfer in the proposed model. The model’s outer surface was excited at multiple excitation frequencies by a sinusoidal heat flux, and the thermal response of the model was presented in the form of thermal images to show the temperature contrast due to the presence of defects. The harmonic approximation method was applied to calculate the phase angle, and its relationship with respect to defect depth and defect size was also studied. The results confirmed that the FEM model has led to a better understanding of lock-in infrared thermography and can be used to detect subsurface defects in the hydroelectric penstock.

Since my country entered the era of Internet economy, the scale of the software industry is gradually expanding, and programming language, as an important tool and idea for software development, is a necessary skill for every development practitioner. Among them, C language, as the basic language of computer software programming, is also an important basic course for cultivating students’ computer application ability in science and engineering majors in colleges and universities. This paper mainly studies the teaching reform of C language programming courses and proposes corresponding optimization measures, so as to lay a solid foundation for the continuous improvement of students’ learning quality of C language courses and the continuous strengthening of programming ability.

According to the World Health Organization (WHO), breast cancer is among the most common cancers worldwide. Most of the anticancer agents have been showing a variety of side effects. Recently, bacterial proteins have been investigated as promising anticancer agents. Azurin is a bacterial cupredoxin protein secreted from Pseudomonas aeruginosa and has been reported as a potent multi-targeting anticancer agent, which makes it an appropriate candidate for drug delivery. Azurin may be delivered to cancer cells using different carriers like polymeric micro and nanoparticles. In the present study, azurin was extracted from the bacterial host and loaded into chitosan particles. Then its effect on MCF-7 cell line was investigated. Chitosan-azurin particles were made using the ion gelation method. Results showed that chitosan-azurin particles are about 200 nm, and the loading of the protein in particles did not affect its integrity. The MTT assay showed a significant reduction in cell viability in azurin and chitosan-azurin-treated cells. The toxicity level after 5 days was 63.78% and 82.53% for free azurin and chitosan-azurin-treated cells, respectively. It seems using an appropriate carrier system for anticancer proteins like azurin is a promising tool for developing low side effect anticancer agents.

In marginalized ecosystem-dependent rural communities, access to ecosystem services plays a crucial role in achieving sustainable livelihoods. This study was conducted to find out the influence of various livelihood capital components on the access mechanism for forest-based Provisioning Services (PS) in some selected villages of the Gosaba Block on the fringes of the Sundarban. The contribution of the livelihood capitals to gain access to Provisioning Services (PS) was identified using factor analysis on 160 households, selected through cluster random sampling. The sustainability levels of livelihood capitals were analyzed using the Prescott-Allen method (2001). The natural, financial, social, and physical capitals were significantly below average, while the human capital was close to average. Enhancement of human, physical, financial, and social capital, ease in issuing Biometric Fisherman cards for entering forests, flexibility in borrowing loans, and ecotourism by involving local villagers must be encouraged to enhance forest-based provisioning services in the near future.