Climate change is one of the most critical global challenges, driven primarily by the rapid increase in greenhouse gas concentrations. Carbon sequestration, the process by which ecosystems capture and store carbon, plays a key role in mitigating climate change. This study investigates the factors influencing carbon sequestration in subtropical planted forest ecosystems. Field data were collected from 100 randomly sampled plots of varying sizes (20 m² × 20 m² for trees, 5 m² × 5 m² for shrubs, and 1 m² × 1 m² for herbs) between February and April 2022. A total of 3,440 plants representing 36 species were recorded, with Prosopis juliflora and Prosopis cineraria as the dominant tree species and Desmostachya bipinnata as the dominant herb. Regression analysis, Pearson correlation, and structural equation modeling were performed using R software to explore relationships between carbon sequestration and various biotic and abiotic factors. Biotic factors such as diameter at breast height (DBH; R=0.94), tree height (R=0.83), and crown area (R=0.98) showed strong positive correlations with carbon sequestration. Abiotic factors like litter (R=0.37), humus depth (R=0.43), and electrical conductivity (E.C; R=0.11) also positively influenced carbon storage. Conversely, pH (R=-0.058), total dissolved solids (TDS; R=-0.067), organic matter (R=-0.1), and nitrogen (R=-0.096) negatively impacted carbon sequestration. The findings highlight that both biotic and abiotic factors significantly influence carbon sequestration in planted forests. To enhance carbon storage and mitigate climate change, efforts such as afforestation, reforestation, and conservation of subtropical forest ecosystems are essential.