The WRKY gene family plays a very diverse role in plant growth and development. These genes contained an evolutionarily conserved WRKY DNA binding domain, which shows functional diversity and extensive expansion of the gene family. In this study, we conducted a genome-wide comparative analysis to investigate the evolutionary aspects of the WRKY gene family across various plant species and revealed significant expansion and diversification ranging from aquatic green algae to terrestrial plants. Phylogeny reconstruction of WRKY genes was performed using the Maximum Likelihood (ML) method; the genes were grouped into seven different clades and further classified into algae, bryophytes, pteridophytes, dicotyledons, and monocotyledons subgroups. Furthermore, duplication analysis showed that the increase in the number of WRKY genes in higher plant species was primarily due to tandem and segmental duplication under purifying selection. In addition, the selection pressures of different subfamilies of the WRKY gene were investigated using different strategies (classical and Bayesian maximum likelihood methods (Data monkey/PAML)). The average dN/dS for each group are less than one, indicating purifying selection. Our comparative genomic analysis provides the basis for future functional analysis, understanding the role of gene duplication in gene family expansion, and selection pressure analysis.

Purpose: The aim of the study is to apply policy analysis matrix (PAM) to identify international competitiveness of marketing channels and policy impacts of government on each marketing channels. Methodology: Policy analysis matrix is employed to evaluate influences of macroeconomic policy on the Tuong-mango value chain. The study investigated 213 sampling observation of eight main actors in chain. Findings: The findings indicate that although domestic channel 4 exhibits competitiveness (Private cost ratio (PRC) < 1), channels 1, 2, and 3 possess both comparative and competitive advantages (PRC < 1, Domestic Resource Cost (DRC) < 1, and social benefit-cost (SBC) > 1). The government’s strategy on production protection, referred to as Nominal protection coefficient on tradable output (NPCO) 0.16, together with the plan for enhancing added value, denoted as Effective protection coefficient (EPC) 0.14 and Subsidy ratio to producers (SRP) −0.18, place a significant emphasis on the first export channel. The government’s subsidy plan grants preferential treatment to Channel 4 in terms of the pricing of commercially available products, with a Nominal protection coefficient on tradable input (NPCI) value of 0.75. A value-added strategy is implemented for export channels 2 and 3, which have EPCs of 0.76 and 0.85, respectively. Policy implications: If the tradable cost is modified by 20%, there will be a change in the ratio of DRC, SBC, EPC, and SRP. While the EPC does not see a 20% reduction in domestic prices, the DRC and SBC do benefit from this cost reduction. A reduction of 20% in the local cost, coupled with a corresponding rise of 20% in the Free on Board (FOB) price, would result in a significant elevation of the SRP for export channels 1, 2, and 3. Conclusion: This is as evidence for the combination of quantitative is a dynamic tool in the policymaking process to ensure targets, constrictions, and consistent policies for agricultural fields. This permits policies to be changed in steps with an alteration in the economy and priorities set up for the tropical fruits and vegetables field.

The holding of soccer events has an important impact on modern urban activities, which is conducive to the economic development, social harmony, cultural integration and regional integration of cities. However, massive energy is consumed during the event preparation and infrastructure construction, resulting in an increase in the city’s carbon emissions. For the sustainable development of cities, it is important to explore the theoretical mechanism and practical effectiveness of the relationship between soccer events and urban carbon emissions, and to adopt appropriate policy management measures to control carbon emissions of soccer events. With the development of green technology, digitalization, and public transportation, the preparation and management methods of soccer events are diversified, and the possibility of carbon reduction of the event is further increased. This paper selects 17 cities in China from 2011 to 2019 and explores the complex impact of soccer events on urban carbon emissions by using green technology innovation, digitalization level and public transportation as threshold variables. The results show that: (1) Hosting soccer events increases carbon emissions with an impact coefficient of 0.021; (2) There is a negative single-threshold effect of green innovation technology, digitalization level and public transportation on the impact of soccer events on carbon emissions, with the impact coefficients of soccer events decreasing by 0.008, 0.01 and 0.06, respectively, when the threshold variable crosses the threshold. These findings will enhance the attention of city managers to the management of carbon emissions from soccer events and provide guidance for reducing carbon emissions from soccer events through green technology innovation, digital means and optimization of public transportation.

The integration of digitalization and servitization has become a significant trend in transforming the manufacturing industry due to digital intelligence technology. This paper examines the impact of the integration of digitalization and servitization on the performance of manufacturing companies and how small-scale enterprises can promote digital transformation leading to servitization. The study involved surveying 331 manufacturing companies in China using a seven-point Likert scale questionnaire. Measurement scales were validated using confirmatory factor analysis and discriminant validity tests. Mediation analysis assessed digitalization’s impact on servitization and firm performance. The study’s findings emphasize the significant impact of digitalization and servitization on enterprises’ performance. Digitalization plays a crucial role in mediating this relationship. The study highlights three critical dimensions of digital variables, including digital technology, digital labor, and digital relationship resources, essential in enabling effective servitization. Manufacturing enterprises generally prefer aligning their technology investments and organizational changes within the digitalization framework to implement servitization successfully. The study suggests two integration strategies, namely conservative and aggressive. The finding emphasizes that the convergence of digitalization and servitization leads to a new manufacturing production mode called digital servitization.

The ultimate objective of the study was to investigate the effects of being landlocked on the living standards in Sub-Saharan African (SSA) countries from 1991 to 2019. Adopting the two-step estimation technique of System GMM (generalized method of moments), the study found that being landlocked has a negative and significant effect on the living standards in SSA countries when using GDP per capita as the living standard measure. Moreover, the historical living standard experiences of SSA countries have a positive and significant influence on the current living standard level. In addition, the population growth rate has a positive and significant effect on the living standards in SSA countries. On the other hand, the official exchange rate, broad money as a percentage of GDP, and inflation have a negative and significant effect on the living standards in SSA countries. Generally, the estimated result reveals the existence of a significant variation in the living standards in landlocked and coastal SSA countries. This study suggests that regional integration between landlocked and transit countries should be improved to minimize entry costs and increase access to global markets for landlocked countries. We argue that this study is of interest to landlocked and coastal countries to increase trade integration and promote the development of both groups, and it will contribute to the scarce empirical evidence.