In this investigation the effect of collection seasons of explants (winter, spring and summer), type of explants (leaf disc and intermodal segments) and length of explants (0.5, 1.0 and 1.5 cm) for callusing in low-chill peach were standardized. The maximum callus induction (97.78%) in the low-chill peach was obtained from the intermodal segments of 0.5 cm in length used as an explant collected during spring season. The structural changes on the surface of the callus (5–7 weeks old yellowish green compact callus) during the progress of somatic embryogenesis of low-chill peach from the both intermodal segment as well as leaf disc derived callus were also examined with the use of scanning electron microscope (SEM). The SEM studies indicated that callus derived from internodal segment explant had the highest frequency of somatic embryos than callus from leaf discs. The SEM investigation, also demonstrated the sequential events/steps leading to low-chill peach somatic embryogenesis which was originating from somatic embryo mother cells through one unicellular pathway. Two types of calli were morphologically distinguished in both leaf disc and intermodal segment generated callus and these were the compact, well organized yellowish green embryogenic callus, containing large number of small, rich cytoplasmic, starch containing meristematic cells and soft and unorganized non-embryogenic callus containing sparsely cytoplasmic, vacuolated, and large cells devoid of metabolic reserves. The present SEM studies clearly demonstrated that somatic cells from peach explants generated callus could develop into fully differentiated somatic embryos through the characteristic embryological patterns of differentiation.

The experiments were carried out to validate an analytical method and to examine the impact of various decontaminating solutions on the removal of acephate residues from okra. Acephate analysis was performed using HPLC-UV, and sample extraction was done using the QuEChERS method. Method validation encompassed assessing specificity, linearity, precision, accuracy, as well as limits of detection (LOD) and quantification (LOQ). The method exhibited excellent linearity with R² values ≥ 0.99. LOD and LOQ were determined at 0.5 µg mL⁻¹ and 2 µg mL⁻¹, respectively. The results indicated average recoveries ranging from 80.2% to 83.3% with a % RSD below 5%. The decontamination procedures include rinsing with running tap water, soaking in lukewarm water, 2% CH₃COOH, 1% NaCl, 5% NaHCO₃, 0.01% KMnO₄, and in commercially available decontamination products such as nimwash, veggie clean, and arka herbiwash for a duration 10 minutes. Among all the treatments, soaking in nimwash solution showed remarkable effectiveness (96.75% removal), followed by veggie clean (94.97% removal) and arka herbiwash (95.80% removal). Washing okra samples in running tap water was found to be the least effective compared to other treatments.

An experiment was carried out to investigate the effect of different organic nutrient solutions and day of harvest on growth parameters, biomass and chemical composition of hydroponically grown sorghum red fodder. The experiment was a 3 × 2 factorial design comprising of 3 nutrient solutions (cattle, poultry and rabbit) and 2 harvesting regimes (8th and 10th day). Cattle, poultry and rabbit dungs were collected fresh and processed into nutrient solutions. Sorghum red seeds were treated, planted on trays, and irrigated twice per day with organic nutrient solution according to the treatments. Growth parameters which were investigated included fodder mat thickness, seedling height, leaf length and width, number of leaves, fresh and dry matter yield; and proximate composition. The results showed that sorghum red fodder irrigated with cattle manure nutrient solution (NS) harvested at 10 days was higher in all, except one (fodder mat thickness) of the growth parameters considered. The crude protein (CP) was highest and similar (P > 0.05) for Poultry NS harvested at 8 and 10 days, and Cattle NS at 10 days (13.13%, 12.67%, and 12.69% respectively). The ash content also favored Cattle NS at 10 days. Cattle NS at 10 days harvest was significantly (P < 0.05) the highest (7.00%), but comparable (P > 0.05) with Rabbit NS at 10 days for NDF. Fresh and DM yields were highest for Cattle harvested at 10 and 8 days respectively. The study recommends Cattle NS as hydroponic organic NS for sorghum red as it enhances fresh and dry matter yields, and nutritive values.

Studies to evaluate the response of passion fruit seedlings in terms of emergence, nursery, and early field growth to growing media and mulching were carried out at the Teaching and Research Farm of Joseph Sarwuan Tarka University Makurdi between July and December 2018. Treatments consisted of five media, composted from readily available substrates. The five nursery media were; medium 1:1:2:3 (SB) composed of top soil + poultry manure + river sand; medium 2:1:2:3 (RHB) – rice hull + poultry manure + river sand; medium 3:2:3:1 (RHB) – rice hull + poultry manure + river sand; medium 4:1:4:3 (SDB) – sawdust + poultry manure + river sand and medium 5:1:2:3 (SDB) – sawdust + poultry manure + river sand. For the nursery experiment, treatments were the five potting media, while the field trial was a 5 × 2 factorial arrangement consisting of the five growing media and mulching status (mulch and no mulch). In both cases, treatments were laid out in randomized designs that were replicated three times. Results showed that there were no significant differences in all the emergence traits evaluated. However, medium M5 (sawdust based) showed superior performance in most of the seedling characters evaluated. Under field conditions, the sawdust based media (M4 and M5) gave the best growth of passion fruit seedlings compared to the other potting media. Application of mulch, however, did not elicit any significant response in plant growth. It is therefore conclusive that sawdust based growing media could be used to produce high quality passion fruit seedlings with the prospect of excellent performance under field conditions.

Horticultural crops are rich in constituents such as proteins, carbohydrates, vitamins, and minerals important for human health. Under biotic and abiotic stress conditions, rhizospheric bacteria are powerful sources of phytohormones such as indole acetic acid (IAA), gibberellic acid (GA), abscisic acid (ABA) and Plant growth regulators including cytokines, ammonia, nitrogen, siderophores, phosphate, and extra cellular enzymes. These phytohormones help horticultural crops grow both directly and indirectly. In recent agricultural practices, the massive use of chemical fertilizers causes a major loss of agricultural land that can be resolved by using the potent plant growth-promoting rhizospheric bacteria that protect the agricultural and horticultural crops from the adverse effect of phytopathogens and increase crop quality and yield. This review highlights the role of multifunctional rhizospheric bacteria in the growth promotion of horticultural crops in greenhouse conditions and agricultural fields. The relevance of plant growth hormones in horticultural crops highlighted in the current study is crucial for sustainable agriculture.

Our study evaluated the effect of vanadium (V) on the behavior of Zinnia elegans “double variegated”. In this experiment, Zinnia plants grown in a greenhouse were fed with a nutrient solution and two concentrations of vanadium (0, 6, and 10 μm) applied four times during the experiment. The V at its levels of 6 µm and 10 µm increased plant length, number of inflorescences and fresh weight. We observed that during the development and appearance of flower buds, and flowering were earlier with the addition of 6 µm and 10 µm. During harvest the changes in size and shape were homogeneous with the control treatment. With the addition of 6 µm, flowers of different sizes were induced, with non-uniform petals, but with different shades of color. With 10 µm the shape of the petals, the distance between them and changes in the shades of the flowers were modified. The postharvest life for the flowers of the control treatment was shorter (15 days), the petals, anthers and floral disc at this time were observed in a poor condition. While 6 µm and 10 µm had a longer postharvest life (20 days), the flowers had a good presentation, their colors were more intense compared to the harvest stage. The application of this beneficial element contributed to the development and flowering of Zinnia in the greenhouse. It is suggested that future research be carried out on the accumulation and/or concentration of vanadium in the different stages of growth or its effect on the concentration of other nutrients.