Cucumis sativus is an important vegetable crop in the world. Agrobacterium mediated transgenic technology is an important means to study plant gene function and variety improvement. In order to further accelerate the transgenic research and breeding process of cucumber, aiming at the Agrobacterium mediated genetic transformation method of cucumber, this paper expounds the research progress and existing problems of Agrobacterium mediated transgenic cucumber from the aspects of influencing factors of cucumber regeneration ability, genetic transformation conditions and various added substances in the process, and prospects the future of improving the efficiency of cucumber genetic transformation and the application of safety screening markers, in order to provide reference for cucumber stress resistance breeding and fruit quality improvement.

In order to explore the preliminary effect of 1-MCP application at seedling stage on the growth effect and yield of open field cucumber, this experiment conducted cultivation experiments on three application periods (leaf spraying at one leaf stage, 2 days before planting, spraying after the third harvest), two treatment times (one treatment, two treatment), and two management methods (removing the first and second female flowers, and conventional management). The results showed that in the open field cucumber cultivation experiment, the application of 1-MCP at seedling stage could promote the growth of cucumber, and the T4 treatment was the best, and the second treatment was better than the first treatment; T4 (0.35 mL 1-MCP + treatment 2 days before colonization + after the third harvest + routine management) treatment scheme had the best effect.

The use of bioproducts, economically viable, are of extreme importance in the protection and stimulation of germination in vegetable crops. This work evaluated the effect of the microorganisms Azospirillum brasiliense, Bacillus sub-tilis, Trichoderma harzianum and the commercial seed treatment product (Fipronil + Pilaclostrobin and Methyl Thiophanate) on seeds and seedlings of lettuce (Lactuca sativa), carrot (Daucus carota) and tomato (Solanum lycopersicum). The seeds were inoculated before being submitted to the germination test. The chemical treatment proved ineffective in protecting the seed of all crops and stimulating germination. T. harzianum increased the germination index of lettuce seeds, had better values in root system size in tomato crop and stimulated radicle emission in carrot. B. subtilis stood out in dry matter accumulation in tomato crop. The microorganisms B. subtilis and T. harzianum present potential for vegetable seed treatment.

Studies related to the use of steel mill slag have become essential, because of the possibility of its use as a component of substrates in the production of seedlings and because this use minimizes the risk of environmental contamination, resulting from inadequate disposal. Thus, the objective of this work was to evaluate the effect of increasing levels of steel slag in substrates composed of soil with tanned bovine manure and sand, on the growth variables and the quality of “Dedo-de-moça” pepper (Capsicum baccatum L.) seedlings. A randomized block design was used with five slag concentrations (0%, 2.5%, 5%, 10% and 20%) and four repetitions. Evaluations occurred at 55 days after sowing, consisting of counting the number of leaves, measuring plant height and collar diameter, quantifying the dry mass of leaves and roots and determining the Dickson Quality Index. Regression models were fitted (P < 0.05) to treatments with increasing levels of steel slag. The addition around 10% of slag to the substrate provided the highest values of growth variables, in seedlings of Dedo-de-moça pepper.

The analysis of the accumulation and export of nutrients by the cowpea crop is fundamental for a more sustainable fertilization program, because the definition of the doses of organic fertilizers based only on the estimated maximum yield does not guarantee the maintenance of soil fertility. The objective of this study was to evaluate the effect of fertilization with chicken manure on the productivity, accumulation and exportation of nutrients by the pods of cowpea. A randomized block design was used, with five doses of chicken manure (0; 5; 10; 20 and 40 t ha^-1) and four repetitions. The highest levels of P and Mg were found in the leaves with the application of 40 t ha^-1 of manure. The maximum pod length was 14.47 cm, estimated with the dose of 33.33 t ha^-1 of manure. The highest values of diameter, number of pods per plant and pod productivity were observed at the highest dose of manure applied. In relative terms, that is, total exported in relation to the total extracted by the aerial part, phosphorus is the nutrient most exported by the pods, on average 58%, followed by N (55%), K (43%), Mg (40%), S (38%) and Ca (17%). At the highest dose, although Ca accumulation occurred in large quantities (31.3 kg ha^-1), only 13% of it was exported by the pods. Fertilizing cowpea with chicken manure supplied essential nutrients and increased pod yield from 7.2 (no fertilization) to 16.3 t ha^-1 (fertilization with 40 t ha^-1 of chicken manure). The plant remains of the cowpea constitute an important source of nutrients, being obtained at the highest dose of manure applied (40 t ha^-1) the following amounts of macronutrients (kg ha^-1): N (51.4); P (5.1); K (27.6); Ca (27.1); Mg (8.2); S (5.1), which may return to the soil, with the incorporation of the plants.

Arbuscular mycorrhizal fungi (AMF) increase the uptake of soluble phosphates, while phosphorus solubilizing fungi (S) promote the solubilization of insoluble phosphate complexes, together benefiting plant nutrition. The use of these organisms in combination with minerals or rocks that provide nutrients is another alternative to maintain crop productivity. The objective of this work was to combine AMF and S with pyroclastic materials (ashes and pumicites) from the Puyehue volcano and phosphoric rocks (PR) from the Río Chico Group (Chubut) and to evaluate the performance of these mixtures as substrates for potted production of Lactuca sativa. To formulate the substrates, a mixture of Ter-rafertil® with ashes was used as a base. Penicillium thomii was the S and spores of the fungus Rhizophagus intraradices (AEGIS® Irriga) served as the source of AMF. Various combinations of microorganisms and the addition or not of RP were evaluated. The treatments were: (1) substrate; (2) substrate + AMF; (3) substrate + S; (4) substrate + AMF + S; (5) substrate: PR; (6) substrate: PR + AMF; (7) substrate: PR + S, and (8) substrate: PR + AMF + S. There were 3 replicates per treatment. The parameters evaluated were total and assimilable P content in the substrate, P in plant tissue and dry biomass. All of them were significantly higher in the plants grown in the substrate added with PR and inoculated with S and AMF. This work confirms that the S/AMF combination with volcanic ashes from Puyehue and PR from Grupo Río Chico formulated with a commercial substrate promote the growth of L. sativa. Thus, it is possible to increase the added value of geomaterials of national origin.

This work aimed to evaluate the effects of using three different substrates in the semi-hydroponic culture of lettuce (Lactuca sativa L.) using two different nutrient solutions. A first trial was performed with a nutrient solution rich in macronutrients and micronutrients suitable for lettuce culture, and a second trial with a nutrient solution with pretreated wastewater from effluents of a cheese factory. The experimental design was in randomized blocks with three repetitions and three substrates were used: perlite, coconut fiber, and expanded clay, in both trials. The following parameters were observed: number of leaves, diameter of the cabbage, fresh and dry weight of the aerial part, chlorophyll index and mineral composition of the lettuce. For the first trial, the highest result for the number of leaves (20 leaves), fresh weight (142.0 g) and dry weight (7.2 g) of the aerial part was obtained in the plants growing on perlite. In the second trial, the highest result for the number of leaves (28 leaves), diameter of cabbage (26.7 cm), fresh weight (118.8 g) and dry weight (9.5 g) of the aerial part were achieved by the plants that were grown in coconut fiber. The nutrient solutions were analyzed after each irrigation cycle to verify the possibility of their discharge into the environment. Several parameters were analyzed: pH, conductivity, redox potential, nitrates, nitrites, ammoniacal nitrogen, chlorides, hardness, calcium, phosphates, sodium, potassium, chemical oxygen demand (COD) and magnesium. Ammoniacal nitrogen was found to be the only nutrient that can limits the discharge of nutrient solutions into the environment. It was also proven that the plants, besides obtaining the nutrients necessary for their development in the semi-hydroponic system with the nutrient solution with pre-treated residual water, also functioned as a purification system, allowing the said nutrient solution to be discharged into the environment at the end of each cycle.

The objective of this work was to evaluate the combined effect of bovine manure, Pseudomonas putida and Trichoderma aureoviride on the development of lettuce (Lactuca sativa). The promotion of plant growth by microorganisms may be a viable and sustainable alternative for lettuce crop management. The experimental design was entirely randomized with five treatments: T0 (witness without fertilization, P. putida and T. aureoviride), TE (cattle manure), TEB (cattle manure + P. putida), TEF (cattle manure + T. aureoviride), TEFB (cattle manure + P. putida + T. aureoviride) and ten repetitions each. The following variables were analyzed: germination velocity index (GVI), first count (FC), germination percentage (GP), leaf area index and productivity. The TEFB treatment proved to be a viable alternative for the production of lettuce, especially for small producers, since all the vegetable production in the region comes from family farming.