Cucumis sativus is an important vegetable crop in the world. Agrobacterium mediated transgenic technology is an important means to study plant gene function and variety improvement. In order to further accelerate the transgenic research and breeding process of cucumber, aiming at the Agrobacterium mediated genetic transformation method of cucumber, this paper expounds the research progress and existing problems of Agrobacterium mediated transgenic cucumber from the aspects of influencing factors of cucumber regeneration ability, genetic transformation conditions and various added substances in the process, and prospects the future of improving the efficiency of cucumber genetic transformation and the application of safety screening markers, in order to provide reference for cucumber stress resistance breeding and fruit quality improvement.

This work aimed to evaluate the effects of using three different substrates in the semi-hydroponic culture of lettuce (Lactuca sativa L.) using two different nutrient solutions. A first trial was performed with a nutrient solution rich in macronutrients and micronutrients suitable for lettuce culture, and a second trial with a nutrient solution with pretreated wastewater from effluents of a cheese factory. The experimental design was in randomized blocks with three repetitions and three substrates were used: perlite, coconut fiber, and expanded clay, in both trials. The following parameters were observed: number of leaves, diameter of the cabbage, fresh and dry weight of the aerial part, chlorophyll index and mineral composition of the lettuce. For the first trial, the highest result for the number of leaves (20 leaves), fresh weight (142.0 g) and dry weight (7.2 g) of the aerial part was obtained in the plants growing on perlite. In the second trial, the highest result for the number of leaves (28 leaves), diameter of cabbage (26.7 cm), fresh weight (118.8 g) and dry weight (9.5 g) of the aerial part were achieved by the plants that were grown in coconut fiber. The nutrient solutions were analyzed after each irrigation cycle to verify the possibility of their discharge into the environment. Several parameters were analyzed: pH, conductivity, redox potential, nitrates, nitrites, ammoniacal nitrogen, chlorides, hardness, calcium, phosphates, sodium, potassium, chemical oxygen demand (COD) and magnesium. Ammoniacal nitrogen was found to be the only nutrient that can limits the discharge of nutrient solutions into the environment. It was also proven that the plants, besides obtaining the nutrients necessary for their development in the semi-hydroponic system with the nutrient solution with pre-treated residual water, also functioned as a purification system, allowing the said nutrient solution to be discharged into the environment at the end of each cycle.

The cambucizeiro (Campomanesia phaea), belonging to the Myrtaceae family, is a native plant of the Brazilian Atlantic Forest. The description of the characteristics of the cambucizeiro fruits is important to support new genetic improvement works and its commercial exploitation, especially regarding the processing of the fruit. The present work aimed to perform the morphological and chemical characterization of the cambucizeiro fruits. Fifty-eight accessions, from different locations in the Atlantic Forest and Serra do Mar in the state of São Paulo, were collected, propagated by seeds and one specimen of each accessory is at the Seedling Production Center in São Bento do Sapucaí (SP). Forty fruits from each access were collected in May and submitted to the following analyses: longitudinal and transversal diameter, total fruit fresh mass, number and mass of seeds, total soluble solids, % citric acid, ratio, firmness, vitamin C and coloration. Fruit conformation varies intensely among accessions. The number of seeds is not a good indicator for the relation with the fruit mass, but the mass of one thousand seeds. Some accessions have high soluble solids content, but, on the other hand, the vast majority have fruits with high acidity. Cambuci is an excellent source of vitamin C. The fruits of the accessions are green in color, persisting an opaque shade when ripe.

Cucumber Variety ‘Drite L108’ (Cucumis sativus L. Cv. Derit L108) was selected as the test material. In the solar greenhouse, different days (1, 3, 5, 7, 9 d) of light (PAR < 200 µmol·m^-2·s^-1) and normal light conditions were designed with shading nets to observe the growth indexes of cucumber plants and the changes of antioxidant enzyme activities in leaves. The results showed that: (1) continuous low light increased the SPAD (relative chlorophyll) value of cucumber leaves and decreased the net photosynthetic rate. The longer the continuous low light days are, the smaller the net photosynthetic rate of cucumber leaves and the worse the photosynthetic recovery ability would be. (2) The plant height, stem diameter and leaf area per plant were lower than CK, and the above indexes could not return to the normal level after 9 days of normal light recovery; the yield and marketability of cucumber fruit decreased under continuous low illumination. (3) The activities of SOD (superoxide dismutase) and POD (peroxidase) in cucumber leaves increased, the activities of CAT (catalase) first increased and then decreased, and the content of MDA (malondialdehyde) continued to increase. The longer the days of continuous light keep, the more seriously the cucumber leaves were damaged by membrane lipid peroxidation. After continuous light for more than 7 days, the metabolic function of cucumber leaves was difficult to recover to the normal level.

The use of bioproducts, economically viable, are of extreme importance in the protection and stimulation of germination in vegetable crops. This work evaluated the effect of the microorganisms Azospirillum brasiliense, Bacillus sub-tilis, Trichoderma harzianum and the commercial seed treatment product (Fipronil + Pilaclostrobin and Methyl Thiophanate) on seeds and seedlings of lettuce (Lactuca sativa), carrot (Daucus carota) and tomato (Solanum lycopersicum). The seeds were inoculated before being submitted to the germination test. The chemical treatment proved ineffective in protecting the seed of all crops and stimulating germination. T. harzianum increased the germination index of lettuce seeds, had better values in root system size in tomato crop and stimulated radicle emission in carrot. B. subtilis stood out in dry matter accumulation in tomato crop. The microorganisms B. subtilis and T. harzianum present potential for vegetable seed treatment.

The objective of this work was to evaluate the effect of potassium concentrations applied via fertigation on the growth, yield and chemical composition of eggplant ‘Ciça’ in a distroferric red Latosol. The treatments were composed of five concentrations of K₂O (0, 36, 72, 108 and 144 kg ha^-1 supplied via fertigation), using potassium chloride as a source, divided into six applications. The irrigation system was of the drip type and irrigation management was done via a “Class A” evaporometer tank. Harvest started at 62 days after transplanting (DAT) and lasted for five months. The variables evaluated were: plant height, number of leaves, fresh fruit mass, number of fruits per plant, yield per plant, productivity and classification of the fruits according to their length and diameter. At 85 DAT, fruit were collected for characterization as to the percentage of lipids, proteins and fibers. Although the potassium fertigation in cover provided a reduction in the production and productivity, the concentrations of 36 kg ha^-1 and 72 kg ha^-1 of K₂O applied via fertigation, increased the physical-chemical characteristics of the fruits.

Arbuscular mycorrhizal fungi (AMF) increase the uptake of soluble phosphates, while phosphorus solubilizing fungi (S) promote the solubilization of insoluble phosphate complexes, together benefiting plant nutrition. The use of these organisms in combination with minerals or rocks that provide nutrients is another alternative to maintain crop productivity. The objective of this work was to combine AMF and S with pyroclastic materials (ashes and pumicites) from the Puyehue volcano and phosphoric rocks (PR) from the Río Chico Group (Chubut) and to evaluate the performance of these mixtures as substrates for potted production of Lactuca sativa. To formulate the substrates, a mixture of Ter-rafertil® with ashes was used as a base. Penicillium thomii was the S and spores of the fungus Rhizophagus intraradices (AEGIS® Irriga) served as the source of AMF. Various combinations of microorganisms and the addition or not of RP were evaluated. The treatments were: (1) substrate; (2) substrate + AMF; (3) substrate + S; (4) substrate + AMF + S; (5) substrate: PR; (6) substrate: PR + AMF; (7) substrate: PR + S, and (8) substrate: PR + AMF + S. There were 3 replicates per treatment. The parameters evaluated were total and assimilable P content in the substrate, P in plant tissue and dry biomass. All of them were significantly higher in the plants grown in the substrate added with PR and inoculated with S and AMF. This work confirms that the S/AMF combination with volcanic ashes from Puyehue and PR from Grupo Río Chico formulated with a commercial substrate promote the growth of L. sativa. Thus, it is possible to increase the added value of geomaterials of national origin.

The use of saline water in agriculture is a viable alternative, considering the increased demand for fresh water. The objective of this study was to evaluate the growth and phytomass production of sugar beet under irrigation with water of different saline concentrations in a field experiment on the campus of the Federal University of Alagoas in Arapiraca. The treatments were five levels of electrical conductivity (1.0, 2.0, 3.0, 4.0 and 5.0 dS m^-1). The design was in randomized blocks, with four repetitions. The maximum yield of sugar beet at 27 days after the application of saline treatments was obtained with a salinity of 3.0 dS m^-1, for the variables plant height (PA), stem diameter (CD), root length (RC), aboveground dry phytomass (FSPA) and total dry phytomass (FST). At 42 days after the application of saline treatments, the variables aboveground fresh phytomass (FFPA), root fresh phytomass (FFR), total fresh phytomass (FFT), aboveground dry phytomass (FSPA) and total dry phytomass (FST) increased with increasing water salinity. Rain may have influenced the results obtained for the evaluations, performed at 42 days after the application of the saline treatments.