Highly nutritive and antioxidants-enriched okra (Abelmoschus esculentus) gets sub-optimal field yield due to the irregular germination coupled with non-synchronized harvests. Hence, the research aimed at assessing the combined impact of seed priming and field-level gibberellic acid (GA₃) foliar spray on the yield and post-harvest quality of okra. The lab studies were conducted using a complete randomized design (CRD), while the field trials were performed following a factorial randomized complete block design (RCBD) with three replications. Okra seeds were subjected to ten different priming methods to assess their impact on seed germination and seeding vigor. In the premier step, okra seeds were subjected to ten different priming methods, like hydro priming for 6, 12, and 18 h, halo priming with 3% NaCl at 35 ℃, 45 ℃, and 60 ℃, acid priming with 80% H₂SO₄ for 2.5, 5, and 10 min. Based on the observation, hydro priming for 12 h exhibited the best germination rate (90%), followed by halo seed priming at 60 ℃ and acid priming for 5 min. Furthermore, the halo priming at 60 ℃ demonstrated the greatest seedling vigor index (1965), whereas acid priming for 5 min resulted in favorable outcomes in terms of early emergence in 2.66 days. In addition, varying concentrations of GA₃ (0, 100, 200, and 300 ppm) were also administered to the best three primed seedlings for evaluating their field performance. The findings indicated that applying GA₃ at a concentration of 300 ppm to seedlings raised through acid priming (80% H₂SO₄ for 5 min) resulted in improved leaf length, reduced time to flowering (first and 50%) and harvest, increased pod diameter, individual pod weight, and yield per plant (735.16 g). Additionally, the treatment involving GA₃ at 300 ppm with halo priming (3% NaCl) at 60 ℃ exhibited the longest shelf life (21 days) of okra with the lowest levels of rotting (6.73%) and color change (1.12) in the polyethylene storage condition.

The coconut industry has deep historical and economic importance in Sri Lanka, but coconut palms are vulnerable to water stress exacerbated by environmental challenges. This study explored using Sunn hemp (Crotalaria juncea L.) in major coconut-growing soils in Sri Lanka to improve resilience to water stress. The study was conducted at the Coconut Research Institute of Sri Lanka to evaluate the growth of Sunn hemp in prominent coconut soils—gravel, loamy, and sandy—to determine its cover crop potential. Sunn hemp was planted in pots with the three soil types, arranged in a randomized, complete design with 48 replicates. Growth parameters like plant height, shoot/root dry weight, root length, and leaf area were measured at 2, 4, 6, and 8 weeks after planting. Soil type significantly impacted all growth parameters. After 8 weeks, sandy soil showed the highest plant height and root length, while loamy soil showed the highest shoot/root dry weight and leaf area, followed by sandy and gravel soils. Nitrogen content at 6 and 8 weeks was highest in loamy soil plants. In summary, Sunn hemp produces more biomass in sandy soils, while loamy soils promote greater nutrient accumulation and growth. This suggests the suitability of Sunn hemp as a cover crop across major coconut-growing soils in Sri Lanka, improving resilience.

Kinnow production is hampered due to the lack of micronutrient applications such as zinc (Zn), iron (Fe), and manganese (Mn), which play a significant role in the metabolic activities of the plant, affecting yield and quality. The farmers of the region use mineral micronutrient fertilizers, but it leads to phytotoxicity due to unoptimized fertilizer application dose. In the present investigation, an attempt has been made to optimize the Zn, Mn, and Fe minerals dose as tank mix foliar application for improvement of fruit yield, quality, and uptake of nutrients. The twelve combinations of different doses of zinc sulphate, manganese sulphate, and ferrous sulphate fertilizers replicated three times were tested at kinnow orchards established at Krishi Vigyan Kendra, Bathinda, Punjab, India. The data revealed that the fruit drop was significantly low in the treatment F₁₂ (43.4%) (tank mix spray of 0.3% ZnSO₄ + 0.2% MnSO₄ + 0.1% FeSO₄ ) compared to control treatment. The fruit yield per tree was significantly higher in the treatment F12 compared to untreated control. The juice percentage was also recorded higher in treatment F12 as compared to control, and the juice percentage improved by 2.6%. The leaf nutrient analysis also revealed translocation of higher amount of nutrient from leaf to fruit under optimized supply of micronutrient. Thus, the application of tank mix spray of 0.3% ZnSO₄ + 0.2% MnSO₄ + 0.1% FeSO₄ may be used for better fruit yield and quality.

The aim of the present study was to determine the effects of single and mixed infections of nematode (Meloidogyne javanica), fungus (Fusarium oxysporum) and bacterium (Xanthomonas axonopodis) on nodulation and pathological parameters of Bambara groundnut (Vigna subterrenea (L.) Verdc.) in field condition. Nematode infested field was used while other pathogens were obtained from diseased plants. The Randomized Complete Block Design (RCBD) was adopted in a 5 × 9 × 5 factorial design (5 blocks, 9 treatments and 5 replicates per treatments) resulting in 225 experimental units. In each experimental unit, three seeds were sown to a depth of 5cm and thinned to one plant per planting hole after germination at day 7. Treatments were inoculated into test plant following standard methods. As a result, the control treatment recorded the highest number of nodules (64.0 ± 6.91), followed by bacterium (45.2 ± 5.11) while N + F + B had the lowest number of root nodules (23.4 ± 2.42). Simultaneous treatment (N + F + B) gave the highest percentage reduction in nodulation (63.44%), followed by treatment N + F7 (56.25%). Fungus treatment recorded the highest mean wilted plants (3.8 + 0.20) followed by N + F7 treatment (3.40 + 0.40). Gall formation in the nematode treatment increased proportionately by 56.33% as the highest recorded, followed by treatment N + F7 with 50.0%. Treatment N + F7 had the highest reproduction factor (Rf) value of 9.30 followed by nematode (8.30), N + B7 (7.40), N + F + B (6.80) and N + F14 (6.50). Zero (0) Rf value was recorded in fungus, bacterium and control treatments. The observed differences in nodulation and pathological parameters among the treatments are significant (P < 0.05). The data provided in this work is important in the control of the three pathogens affecting the productivity of Bambara nut. Formulation of a single protectant should be designed to have potent effects on the three pathogens to achieve effective protection and good production of Bambara nut.

In higher eukaryotes, the genes’ architecture has become an essential determinant of the variation in the number of transcripts (expression level) and the specificity of gene expression in plant tissue under stress conditions. The modern rise in genome-wide analysis accounts for summarizing the essential factors through the translocation of gene networks in a regulatory manner. Stress tolerance genes are in two groups: structural genes, which code for proteins and enzymes that directly protect cells from stress (such as genes for transporters, osmo-protectants, detoxifying enzymes, etc.), and the genes expressed in regulation and signal transduction (such as transcriptional factors (TFs) and protein kinases). The genetic regulation and protein activity arising from plants’ interaction with minerals and abiotic and biotic stresses utilize high-efficiency molecular profiling. Collecting gene expression data concerning gene regulation in plants towards focus predicts an acceptable model for efficient genomic tools. Thus, this review brings insights into modifying the expression study, providing a valuable source for assisting the involvement of genes in plant growth and metabolism-generating gene databases. The manuscript significantly contributes to understanding gene expression and regulation in plants, particularly under stress conditions. Its insights into stress tolerance mechanisms have substantial implications for crop improvement, making it highly relevant and valuable to the field.